Title:
【発明の名称】タイセイヨウサケ由来の孵化液の抽出方法および使用
Document Type and Number:
Japanese Patent JP2001526026
Kind Code:
A
Abstract:
A procedure is described for obtaining valuable endoproteases (or zonases) from hatchery-produced Atlantic salmon eggs. Synchronized hatching by for instance elevated temperature, is followed by filtration through cheese cloth. The filtrate (hatching fluid) may be stored for months or year (depending on conditions) without loss of activity. Extraneous matters are removed by centrifugation (16,000 g, 2x15 min) after addition of urea (2 or 4 M, or more). High purity zonases are obtained by simple chromatographic procedures (gel filtration, affinity chromatography, isoelectric focusing), yielding sequence-grade purity after all three steps are performed in sequence. All preparations of salmon zonases exhibit valuable enzymatic properties with regard to proteolysis, both in terms of catalysis and stability.
Inventors:
Walther, Bernd Tehoe.
Ron, Jun Jun Jay.
Ron, Jun Jun Jay.
Application Number:
JP2000524409A
Publication Date:
December 18, 2001
Filing Date:
December 11, 1998
Export Citation:
Assignee:
Aqua bio technology ass
International Classes:
C12N9/50; C12N9/64; (IPC1-7): C12N9/50
Attorney, Agent or Firm:
Takashima Hajime