Title:
【発明の名称】ヘモグロビン及び細胞の分析のための組成物及び方法
Document Type and Number:
Japanese Patent JP2002507749
Kind Code:
A
Abstract:
A cyanide-free lytic reagent composition and method for measuring the total hemoglobin concentration in a blood sample, for counting the number of leukocytes and for deferential counting of leukocyte subpopulations are described. The cyanide-free lytic reagent composition cotains a quaternary ammonium salt or a pyridinium salt to lyse erythrocytes and release hemoglobin, and an organic ligand including triazole and its derivatives, tetrazole and its derivatives, alkaline metal salts of oxonic acid, melamine, aniline-2-sulfonic acid, quinaldic acid, 2-amino-1,3,4-thiadiazole, triazine and its derivatives, urazole, DL-pipecolinic acid, isonicotinamide, anthranilonitrile, 6-aza-2-thiothymine, 3-(2-thienyl)acrylic acid, benzoic acid and alkali metal and ammonium salts of benzoic acid, and pyrazine and its derivatives to form a stable chromogen with hemoglobin, and a salt to adjust conductivity of the reagent for impedance measurement. The reagent composition is mixed with a blood sample without pre-dilution and the UV absorption of the sample mixture is measured at the predetermined absorption wavelength. Counting the number of leukocytes and differential counting of leukocyte subpopulations are accomplished simultaneously on an automated cell counter utilizing DC impedance measurement.
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Inventors:
Lee
Young, Carroll
Young, Carroll
Application Number:
JP2000538237A
Publication Date:
March 12, 2002
Filing Date:
March 16, 1999
Export Citation:
Assignee:
Coulter International Corporation
International Classes:
G01N33/48; G01N33/49; G01N33/50; G01N33/569; G01N33/72; (IPC1-7): G01N33/72; G01N33/48; G01N33/49
Attorney, Agent or Firm:
Takashi Ishida (4 others)