Title:
【発明の名称】高い特異性を有するプライマー、増幅法およびキット
Document Type and Number:
Japanese Patent JP2003500038
Kind Code:
A
Abstract:
For nucleic acid amplification including extension of primers by a DNA polymerase, high specificity primers are provided. The primers include a type of hairpin structure in which a single-stranded loop separates complementary 3' and 5' arms and in which the loop and the 3' arm are complementary to the target nucleic acid. Amplification methods, assays and kits including such primers are included in the invention.
Inventors:
Tiagi, Sanjay
Clammer, Fred, Earl.
Robert, Vertician
Clammer, Fred, Earl.
Robert, Vertician
Application Number:
JP2000619817A
Publication Date:
January 07, 2003
Filing Date:
May 03, 2000
Export Citation:
Assignee:
The Public Health Research Institute of the City of New York Incorporated
International Classes:
C12N15/09; C07H21/00; C07H21/02; C07H21/04; C12P19/34; C12Q1/48; C12Q1/68; C12Q1/6818; C12Q1/6844; G01N33/00; G01N33/53; (IPC1-7): C12N15/09; C12Q1/48; C12Q1/68
Attorney, Agent or Firm:
Yusuke Hiraki (2 outside)