Title:
【発明の名称】マルチウェルにおける減圧濾過による自動タンパク質精製
Document Type and Number:
Japanese Patent JP2003506456
Kind Code:
A
Abstract:
The invention relates to a process and a reagent kit for obtaining clear solutions containing cell contents from biological samples in a high throughput process, comprising the steps of preparing a plurality of protein-containing solutions which contain insoluble ingredients, in separate chambers of a multi-chamber filtration unit, eliminating insoluble ingredients by filtering the solutions through the multi-chamber filtration unit with the application of a pressure differential, while preventing cross contamination between adjacent chambers by chemical and/or mechanical means, and collecting the individual filtrates separately in collecting containers, as well as a process for recovering cell contents from the clear solutions obtained. The invention further relates to the use of an apparatus for recovering clear protein-containing solutions from biological samples.
Inventors:
Ribe Joachim
Scheffer Frank
Steinert Kerstin
Lübenau Helge
Scheffer Frank
Steinert Kerstin
Lübenau Helge
Application Number:
JP2001515694A
Publication Date:
February 18, 2003
Filing Date:
August 02, 2000
Export Citation:
Assignee:
Qiagen Gezel Shaft Mitt Beschlenktel Haftung
International Classes:
B01D19/04; B01D29/01; B01D61/18; B01L3/00; B01L9/06; C07K1/14; C07K1/34; C07K1/36; B01J20/281; C12M1/32; C12N9/10; C12N15/10; C12Q1/02; G01N1/34; G01N30/88; (IPC1-7): C07K1/14; C07K1/34; C12M1/32; C12N9/10; C12Q1/02; G01N30/48; G01N30/88
Domestic Patent References:
| JPH04227032A | 1992-08-17 | |||
| JPH02187110A | 1990-07-23 | |||
| JPH07501222A | 1995-02-09 |
Foreign References:
| US4902481A | 1990-02-20 | |||
| WO1997008306A1 | 1997-03-06 |
Other References:
JPN7010003418, ＱＩＡＧＥＮ， ＱＩＡａｍｐ９６ ＤＮＡ Ｂｌｏｏｄ ＢｉｏＲｏｂｏｔ Ｋｉｔ Ｈａｎｄｂｏｏｋ, 199905, Ｐ．１−２３
Attorney, Agent or Firm:
Toshio Shiozawa (2 outside)