Title:
【発明の名称】トランスジェニック家禽を構築する方法
Document Type and Number:
Japanese Patent JP2003508068
Kind Code:
A
Abstract:
A method of transgenic fowl construction using germline spermatogonial cells for transfer of genetic information in fowl strain, which method is carried out so that the testicles only of an acceptor cock are irradiated with gamma rays up to the absorbed dose 8 Gy in one irradiation repeatedly and externally. Thereby, the original germline spermatogonial cells in the testicles of the acceptor cock are destroyed, which acceptor cock is then not able to produce sperms, whereby, the testicles structure, including the Sertoli's and Leydig's cells remains preserved for implantation of foreign germline spermatogonial cells of a donor cock. The new implanted germline spermatogonial cells then continue in production of sperms. The sperms are able to fertilize and that store all genetic information of the germline spermatogonial cells of the donor cock. The implantation is carried out after 50 days and more after the last irradiation.
Inventors:
Trefill, pavel
Kotorbova, Arena
Kotorbova, Arena
Application Number:
JP2001521147A
Publication Date:
March 04, 2003
Filing Date:
September 08, 2000
Export Citation:
Assignee:
Biopharm, Biz Kumny Ustaf Biopharmacy A. Veterinalni Tsehar Letie A.S.
International Classes:
C12N15/90; A01K67/00; A01K67/027; C12N15/09; (IPC1-7): A01K67/027; C12N15/09
Attorney, Agent or Firm:
Takashi Ishida (4 others)