Title:
【発明の名称】タンパク質又はペプチドをリポソームに封入するための方法及びこの方法で調製されたリポソーム及びその使用
Document Type and Number:
Japanese Patent JP2003513901
Kind Code:
A
Abstract:
The invention relates to a novel method for encapsulating proteins or peptides in liposomes which has favorable encapsulation rates of > 30 %. The invention also relates to the liposomes produced using this method, and to their use. The method for encapsulating proteins or peptides in liposomes is characterized in that: the protein or peptide to be encapsulated is dissolved in an aqueous buffer medium; solutions of membrane-forming substances are added, which are selected such that the resulting membrane enters into a reversible mutual reaction with the protein or peptide; the protein or peptide, which is located on the outside of the formed liposome membrane, is detached, and; the liposomes are extracted from the solution.
Inventors:
Pantsner, stephen
Application Number:
JP2001536115A
Publication Date:
April 15, 2003
Filing Date:
November 09, 2000
Export Citation:
Assignee:
Novosom Game Behr
International Classes:
A61K9/127; A61K38/46; A61K47/12; A61K47/18; A61K47/20; A61K47/24; A61K47/28; A61K47/44; A61P17/00; (IPC1-7): A61K9/127; A61K47/12; A61K47/18; A61K47/20; A61K47/24; A61K47/28; A61K47/44
Domestic Patent References:
| JPH0717874A | 1995-01-20 |
Foreign References:
| WO1997029769A1 | 1997-08-21 | |||
| WO1999017740A1 | 1999-04-15 |
Attorney, Agent or Firm:
Yusuke Hiraki (2 outside)