Title:
ヘテロ二本鎖の相補性を増大させる方法
Document Type and Number:
Japanese Patent JP2005507641
Kind Code:
A
Abstract:
We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
Inventors:
Paget, Haru Es.
Fitz Morris, Wayne P.
Lindbow, John A.
Fitz Morris, Wayne P.
Lindbow, John A.
Application Number:
JP2002578470A
Publication Date:
March 24, 2005
Filing Date:
February 01, 2002
Export Citation:
Assignee:
Large Scale Biology Corporation
International Classes:
C12N15/09; C12N15/10; C12P21/00; (IPC1-7): C12N15/09; C12P21/00
Domestic Patent References:
| JP2004509628A | 2004-04-02 | |||
| JPH09504437A | 1997-05-06 | |||
| JP2004509628A | 2004-04-02 | |||
| JPH09504437A | 1997-05-06 |
Foreign References:
| WO1999029902A1 | 1999-06-17 | |||
| WO2000000632A1 | 2000-01-06 | |||
| WO2000046344A2 | 2000-08-10 | |||
| WO1999041368A2 | 1999-08-19 | |||
| WO1999029902A1 | 1999-06-17 | |||
| WO2000000632A1 | 2000-01-06 | |||
| WO2000046344A2 | 2000-08-10 | |||
| WO1999041368A2 | 1999-08-19 |
Other References:
JPN7008004487, Ｊ．Ｍｏｌ．Ｂｉｏｌ．， １９９３， Ｖｏｌ． ２３０， ｐ． ８６８−８７７
JPN7008004488, Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ， １９９８， Ｖｏｌ． ２６， Ｎｏ． ２０， ｐ． ４５９７−４６０２
JPN7008004488, Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ， １９９８， Ｖｏｌ． ２６， Ｎｏ． ２０， ｐ． ４５９７−４６０２
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Tetsuji Koga
Yoichi Watanabe
Masaya Nishiyama
Takashi Ishida
Tetsuji Koga
Yoichi Watanabe
Masaya Nishiyama