Title:
RNA転写反応を改善する方法
Document Type and Number:
Japanese Patent JP2006506962
Kind Code:
A
Abstract:
Methods are described for eliminating single-stranded oligonucleotides from a sample prior to RNA transcription, thereby reducing non-template derived production of RNA. In one embodiment, a sample containing the template for RNA transcription is treated with one or more exonucleases to remove single-stranded oligonucleotides from the reaction mixture prior to RNA transcription. In another embodiment, the sample containing the template for RNA transcription is contacted with an oligonucleotide complementary to the single-stranded oligonucleotide present in the sample, and allowed to hybridize to form double-stranded oligonucleotides.
Inventors:
Cam, frederick curl
Zoo, Jessica Wye
Zoo, Jessica Wye
Application Number:
JP2004510479A
Publication Date:
March 02, 2006
Filing Date:
May 30, 2003
Export Citation:
Assignee:
JANSSEN PHARMACEUTICA NAAMLOZE VENNOOTSCHAP
International Classes:
C12N15/09; C12Q1/68
Attorney, Agent or Firm:
Heikichi Odashima