Title:
タンパク質の折り畳み方法
Document Type and Number:
Japanese Patent JP2009529571
Kind Code:
A
Abstract:
The present invention concerns a method for folding a Transforming Growth Factor Beta, or a functional analogue thereof, into a dimeric, biologically active form. The method involves adding solubilized, unfolded monomeric growth factor to a solution containing 2-(cyclohexylamino)-ethanesulfonic acid (CHES) or a functional analogue thereof and a low molecular weight sulfhydryl/disulfide redox system. The solution is then incubated under conditions suitable for generating dimeric biologically active Transforming Growth Factor Beta.
Inventors:
Ferguson, Mark, William, James
Mellows, philip
Loverty, Hugh, Gerald
Oxleston, Nick
Okane, sharon
Higgin Bottom, Simmon
Mellows, philip
Loverty, Hugh, Gerald
Oxleston, Nick
Okane, sharon
Higgin Bottom, Simmon
Application Number:
JP2008558878A
Publication Date:
August 20, 2009
Filing Date:
March 09, 2007
Export Citation:
Assignee:
Lenovo Limited
International Classes:
C07K1/113; A61P1/16; A61P9/00; A61P17/00; A61P17/02; A61P19/00; A61P27/02; A61P43/00; C07K14/495; C12N15/09; C12P21/02; A61K38/00
Domestic Patent References:
| JPH03191791A | 1991-08-21 | |||
| JP2001519149A | 2001-10-23 |
Other References:
JPN6012026414; ＥＪＩＭＡ Ｄ ｅｔ ａｌ．， Ａ ｎｏｖｅｌ "ｒｅｖｅｒｓｅ ｓｃｒｅｅｎｉｎｇ" ｔｏ ｉｄｅｎｔｉｆｙ ｒｅｆｏｌｄｉｎｇ ａｄｄｉｔｉｖｅｓ ｆｏｒ ａｃｔｉｖｉｎ-Ａ， Ｐｒｏｔｅｉｎ Ｅ
Attorney, Agent or Firm:
Sonoda Yoshitaka
Kobayashi Yoshinori
Kobayashi Yoshinori