Title:
新規血栓溶解分子及びその製造法
Document Type and Number:
Japanese Patent JP2009536821
Kind Code:
A
Abstract:
New thrombolytic protein molecules such as recombinant staphylokinase or streptokinase, urokinase, tissue plasminogen activator and the like, and suitable variants thereof, for targeting to brain tissue or any other tissue by either fusing to, or by synthesizing the candidate thrombolytic molecule(s) with a protein sequence comprising a strong amphipathic alpha helix containing protein transduction domain. Thrombolytic protein molecule(s) so engineered with the protein transduction domain is useful for enhanced uptake of such protein thrombolytic molecule(s) across the cell membranes and tissues including the blood brain barrier and find their use in the treatment of vascular thrombosis including cerebrovascular disorders caused by cerebral thrombosis or cerebral haemorrhage when used a as a therapeutic. The design and processes for cloning, expression, purification and protein transduction of such proteins across cell membranes.
Inventors:
Ella Krishna Mercy
Smarty kandaswami
Smarty kandaswami
Application Number:
JP2009508677A
Publication Date:
October 22, 2009
Filing Date:
May 11, 2007
Export Citation:
Assignee:
Bahara Biotech International Limited
International Classes:
C12N15/09; A61K38/00; A61P7/02; A61P9/10; C12N9/12
Foreign References:
| WO2006029078A2 | 2006-03-16 | |||
| WO2002102417A1 | 2002-12-27 | |||
| WO2005014625A1 | 2005-02-17 | |||
| WO2004110359A2 | 2004-12-23 |
Other References:
JPN6012036206; Ｓｃｉｅｎｃｅ，１９９９ Ｓｅｐ ３，２８５（５４３３），ｐ．１５６９-７２
Attorney, Agent or Firm:
Masaki Hirota