Title:
LIGHT-ACTIVATED CHIMERIC OPSINS AND METHODS OF USING THE SAME
Document Type and Number:
Japanese Patent JP2019205455
Kind Code:
A
Abstract:
To provide compositions comprising chimeric light-activated protein cation channels which are capable of mediating a depolarizing current in a cell when the cell is illuminated with light.SOLUTION: Provided are animal cells comprising a light-activated protein expressed on a cell membrane, the protein being (a) a chimeric protein derived from VChR1 from Volvox carteri and ChR1 from Chlamydomonas reinhardti, which protein comprises the amino acid sequence of VChR1 having at least the first and second transmembrane helices replaced by the first and second transmembrane helices of ChR1; (b) responsive to light; and (c) capable of mediating a depolarizing current in a cell when the cell is illuminated with light.SELECTED DRAWING: Figure 1a-b
Inventors:
KARL DEISSEROTH
OFER YIZHAR
LIEF FENNO
PETER HEGEMANN
MATTHIAS PRIGGE
OFER YIZHAR
LIEF FENNO
PETER HEGEMANN
MATTHIAS PRIGGE
Application Number:
JP2019137443A
Publication Date:
December 05, 2019
Filing Date:
July 26, 2019
Export Citation:
Assignee:
UNIV LELAND STANFORD JUNIOR
International Classes:
C12N5/10; A61K38/17; A61P25/00; A61P25/18; A61P25/28; C12N15/12; C12N15/62; C12N15/85
Domestic Patent References:
| JP2006217866A | 2006-08-24 |
Foreign References:
| WO2009131837A2 | 2009-10-29 | |||
| WO2009119782A1 | 2009-10-01 |
Other References:
LIN, J.Y., EXP. PHYSIOL., vol. 96, no. 1, JPN6015036547, 9 July 2010 (2010-07-09), pages 19 - 25, ISSN: 0004465811
WANG, H. ET AL., J. BIOL. CHEM., vol. 284, no. 9, JPN6015036546, 27 February 2009 (2009-02-27), pages 5685 - 5696, ISSN: 0004465812
LIN JY ET AL., BIOPHYS. J., vol. 96, no. 5, JPN6010050669, 4 March 2009 (2009-03-04), pages 1803 - 1814, ISSN: 0004326419
KIANIANMOMENI A ET AL., PLANT PHYSIOL., vol. 151, no. 1, JPN6010050677, September 2009 (2009-09-01), pages 347 - 366, ISSN: 0004326420
WANG, H. ET AL., J. BIOL. CHEM., vol. 284, no. 9, JPN6015036546, 27 February 2009 (2009-02-27), pages 5685 - 5696, ISSN: 0004465812
LIN JY ET AL., BIOPHYS. J., vol. 96, no. 5, JPN6010050669, 4 March 2009 (2009-03-04), pages 1803 - 1814, ISSN: 0004326419
KIANIANMOMENI A ET AL., PLANT PHYSIOL., vol. 151, no. 1, JPN6010050677, September 2009 (2009-09-01), pages 347 - 366, ISSN: 0004326420
Attorney, Agent or Firm:
Naoto Uchida