Title:
【発明の名称】エンドトキシンの測定法
Document Type and Number:
Japanese Patent JP2957251
Kind Code:
B2
Abstract:
A method of assaying endotoxin accurately to thereby allow a definite diagnosis of gram negative bacterial sepsis, which has been very difficult heretofore, to be conducted rapidly, by adding an aqueous solution of a mixture of a specified surfactant, a compound containing an imidazolyl or amino group, an alkaline earth metal salt and an alkali metal hydroxide to a specimen and then adding a limulus amoebocyte lysate component thereto. An excellent specimen solution can be prepared because an endotoxin adherent to protein, lipid, platelet, etc., can be liberated efficiently merely by adding the aqueous solution which need not be subjected to various operations including separation. Therefore this method serves to solve the problem of the prior art method of assaying endotoxin by the limulus test using plasma, serum, etc., as a specimen that it necessitates pretreatment such as centrifugal separation of denatured precipitates formed by an acid treatment, so that the operation is complicated.
Inventors:
TANAKA SHIGENORI
TAMURA HIROSHI
TAMURA HIROSHI
Application Number:
JP25741590A
Publication Date:
October 04, 1999
Filing Date:
September 28, 1990
Export Citation:
Assignee:
SEIKAGAKU KOGYO KK
International Classes:
G01N33/579; (IPC1-7): G01N33/579
Domestic Patent References:
| JP5551340A | ||||
| JP5127994A |
Attorney, Agent or Firm:
Hajime Tsukuni (1 person outside)