Title:
【発明の名称】塩基性線維芽細胞成長因子蛋白質の結晶およびその製造法
Document Type and Number:
Japanese Patent JP3032843
Kind Code:
B2
Abstract:
PURPOSE:To obtain the subject crystals, remarkably improved in purity and stability and useful as a remedy for wounds, etc., by dissolving a basic fibroblastic growth factor (bFGF) protein in a solvent consisting essentially of water in a high concentration, adding a water-soluble substance thereto and depositing the crystals. CONSTITUTION:An expression vector containing a DNA capable of coding a bFGF protein is introduced into Escherichia coli, etc., which is then transformed. The resultant transformant is cultured to prepare a bFGF protein, which is subsequently dissolved in a solvent consisting essentially of water in a high concentration. A water-soluble substance (e.g. polyethylene glycol) is then added to the obtained solution and regulated to pH6.0 with an aqueous solution of sodium citrate, etc. The regulated solution is subsequently allowed to stand for 4 days to deposit crystals. Thereby, the objective crystals of the bFGF protein are obtained.
Inventors:
Satoshi Fujishima
Tsunehiko Fukuda
Tsunehiko Fukuda
Application Number:
JP4409690A
Publication Date:
April 17, 2000
Filing Date:
February 23, 1990
Export Citation:
Assignee:
Takeda GmbH
International Classes:
C12P21/00; A61K38/00; A61K38/27; A61P17/00; A61P43/00; C07K14/435; C07K14/50; C07K14/52; C30B29/58; C12R1/19; (IPC1-7): C07K14/50; A61K38/27; A61P17/00; A61P43/00; C12P21/00
Domestic Patent References:
| JP63307898A | ||||
| JP2193A | ||||
| JP58164597A |
Other References:
【文献】欧州公開297262(EP,A1)
【文献】Nature,Vol.249,p123(1974)
【文献】Nature,Vol.249,p123(1974)
Attorney, Agent or Firm:
Shuichi Takahashi (2 outside)