Title:
不連続プライマー伸長による核酸の反復配列長の決定
Document Type and Number:
Japanese Patent JP3509025
Kind Code:
B2
Abstract:
In a first aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer extension reagent; separating the target-primer hybrid and unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent; measuring a signal produced by the label; treating the label so as to render the label undetectable; and repeating the above steps until the signal is substantially less than a signal detected in a previous cycle. In a second aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer-extension reagent; separating the target-primer hybrid from unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent and with a primer termination reagent, the primer termination reagent including a nucleotide terminator having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent and unreacted primer termination reagent; measuring a signal produced by the label; and repeating the above steps until a signal is detected indicating incorporation of the nucleotide terminator. The invention further includes kits useful for practicing the above methods.
Inventors:
ブラスバンド，アンドリュー ジェイ．
リバク，ケニース ジェイ．
ロウ，アダム エル．
リバク，ケニース ジェイ．
ロウ，アダム エル．
Application Number:
JP50071099A
Publication Date:
March 22, 2004
Filing Date:
May 11, 1998
Export Citation:
Assignee:
アプレラ コーポレイション
International Classes:
C12Q1/68; C12N15/09; (IPC1-7): C12Q1/68; C12N15/09
Attorney, Agent or Firm:
山本 秀策