Title:
レクチン経路欠損検定
Document Type and Number:
Japanese Patent JP4347808
Kind Code:
B2
Abstract:
The invention refers to an in vitro method of functionally determining at physiological conditions deficiencies in the lectin pathway of the complement system, the method comprising the steps of (a) providing a sample of mammalian blood, serum, plasma or another body fluid; (b) preventing in the sample the activation of the classical pathway by contacting the sample with an inhibitor of a molecule of the C1 complex of the complement system; (e) preventing in the sample the activation of the alternative pathway; (d) activating the lectin pathway in the sample; and (e) determining in the sample any activation of the autologous C5b-9 complex. The invention also refers to a kit for functionally determining in a body fluid from a mammal deficiencies in the lectin pathway of the complement system, which kit comprises the separate items (a) an inert carrier and a substance activating the lectin pathway; (b) a diluent comprising an inhibitor of a molecule of the C1 complex; and an antibody against the autologous C5b-9 complex.
Inventors:
Roast, Johanna
Daha, Mohamed El.
Bouman, Lee Har.
Hack, Cornelis Erik
Daha, Mohamed El.
Bouman, Lee Har.
Hack, Cornelis Erik
Application Number:
JP2004524435A
Publication Date:
October 21, 2009
Filing Date:
July 11, 2003
Export Citation:
Assignee:
Euro Diagnostica Actie Volague
International Classes:
G01N33/53; G01N33/543; G01N33/564; G01N33/68; C07K5/078; C07K7/06; C07K7/08
Other References:
ZIMMERMANN-NIELSEN E. et al.,"Complement activation mediated by mannan-binding lectin in plasma from healthy individuals and from patients with SLE, crohn's disease and colorectal cancer. Suppressed activation by SLE plasma.",Scand. J. Immunol.,2002年 1月,Vol.55,P.105-110
堀内孝彦、塚本浩,「II.膠原病検査の進歩と病態解明 7.補体」,日本内科学会雑誌,1998年12月,Vol.87, No.12,P.2427-2433
藤田禎三、 松下操,「感染防御の分子機構に関する最近の進歩 感染防御における補体レクチン経路の役割」,臨床免疫,2001年,Vol.35, No.1,P.38-43
堀内孝彦、塚本浩,「II.膠原病検査の進歩と病態解明 7.補体」,日本内科学会雑誌,1998年12月,Vol.87, No.12,P.2427-2433
藤田禎三、 松下操,「感染防御の分子機構に関する最近の進歩 感染防御における補体レクチン経路の役割」,臨床免疫,2001年,Vol.35, No.1,P.38-43
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Ayako Okamura
Takashi Ishida
Fukumoto product
Tetsuji Koga
Ayako Okamura