Title:
微生物組換酵素を利用した色素体の形質転換方法
Document Type and Number:
Japanese Patent JP4472344
Kind Code:
B2
Abstract:
The objective of this invention is to enhance the efficiency of plastid transformation using nuclear transformed plants in which the microbial recombinase A(recA) is to target to (or expressed in) the plastid. This invention will be better explained by the following detailed descriptions. A plant is transformed with a nuclear transformation vector containing the microbial recA gene added with a plastid targeting sequence. In this nuclear transformed plant, the frequency of plastid transformation is enhanced greater than two-folds due to increased homologous recombination between the plastid transformation vector carrying genes of interest (or target genes) and the plastid genome. In addition, because plastid transformation is accomplished through a gradual process, adventitious shoots selected after being subjected to plastid transformation should be cut into explants, and then shoots regenerated from the explants are to be reselected until all of the plastids in the shoots are uniformly transformed. However, when the nuclear transformed plant is used, the number of reselection is reduced to ½ to 1/3 due to increased homologous recombination.
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Inventors:
Riu, Jean-Ryeol
Geoun, Won-June
Min, San-Ran
Geoun, Seok-won
Han Soo Kyung
Geoun, Won-June
Min, San-Ran
Geoun, Seok-won
Han Soo Kyung
Application Number:
JP2003560221A
Publication Date:
June 02, 2010
Filing Date:
December 31, 2002
Export Citation:
Assignee:
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
International Classes:
A01H1/00; C12N15/09; C12N15/82
Foreign References:
| WO2000028008A1 |
Other References:
日本農芸化学会誌,1997年,Vol. 71, No. 12,pp. 1295-1298
Proceedings of the National Academy of Sciences of the United States of America,1993年,Vol. 90,pp. 913-917
Proceedings of the National Academy of Sciences of the United States of America,1993年,Vol. 90,pp. 913-917
Attorney, Agent or Firm:
▲吉▼川 俊雄