肝細胞成長因子のN末端フラグメントを精製するための方法

Japanese Patent JP4511589

B2

A method for the production of the N-terminal four kringle-containing fragment of hepatocyte growth factor (NK4) by expression of a nucleic acid encoding said NK4 in a microbial host cell, isolation of inclusion bodies containing said NK4 in denatured form, solubilization of the inclusion bodies and naturation of the denatured NK4, characterized in that solubilization and naturation are performed at pH 7-9 in phosphate buffered solution, provides NK4 in high purity and high yield.

JP7065772	Nucleic acid encoding a repetitive amino acid sequence rich in proline and alanine residues with a low repetitive nucleotide sequence
WO/1995/028488	NON-SPLICING VARIANTS OF gp350/220
WO/2002/036785	PROTEIN COMPLEXES AND PROCESS FOR PRODUCING THE SAME

Hesse, Friederike
Lanzendelfer, Martin
Papadimitryou, Apollon
Stracke, Yang

JP2007500187A

July 28, 2010

March 02, 2005

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F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT

C12P21/02; C07K1/14; C07K1/20; C07K14/475; C12N15/09

JP2000517172A
JP2001500531A

J. Biol. Chem., (2003), Vol. 278, No. 39, p. 37400-37408
Biochem. J., (2001), Vol. 358, p. 263-268

Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Kazuhiro Nakamura
Masaru Nakajima

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