Title:
液体試料を分析するための方法及びシステム
Document Type and Number:
Japanese Patent JP4607899
Kind Code:
B2
Abstract:
A reaction loop (42) made of a transparent tube around which the means of detection (41) are coupled, is filled with a minimal volume of the sample to be analyzed. A fixed volume of at least one reactant is injected into the reaction loop and the amount of light filtered is detected using the means of detection. The reactants are then evacuated from the reaction loop. A concentration gradient is detected in the reaction loop, which is a transparent capillary or microfluid channel. The reactants are evacuated from the reaction loop is done using the remaining sample or the next sample, or the flow of sample is not interrupted to allow continuous analysis. Fixed volumes of reactant are injected at predefined time intervals, with a flow rate of the order of 10-1000 mu L/minute after a waiting time. A linear detection is carried out along the reaction loop to obtain a spatial and temporal relationship for the reactions. Alternatively a momentary detection is carried out in one place in the loop to obtain a temporal relationship for the reactions at one point. In this case a momentary detector is used which can be moved along the reaction loop. An independent claim is made for an analysis system to carry out the above process, with an inlet (E) for the sample; a transparent pipe (42); a syringe (43) connected to the reaction loop to deliver doses of reactant; means of lighting and means of detecting the amount of light transmitted by the sample after filtering. The means of detection are a battery of diodes, or two fibre optics placed on either side of the reaction loop. The system includes a peristaltic pump (40) to introduce the sample and a micro- valve upstream of the sample inlet. A T-shaped branch is connected respectively to the sample inlet (E), the syringe (43) and the reaction loop (42).
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Inventors:
Magnard, Arastere
Davan, Thierry
Davan, Thierry
Application Number:
JP2006544518A
Publication Date:
January 05, 2011
Filing Date:
December 15, 2004
Export Citation:
Assignee:
INSERM(INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE)
International Classes:
G01N35/08; G01N1/00; G01N21/79
Domestic Patent References:
| JP2002500765A | ||||
| JP55029791A | ||||
| JP7083935A | ||||
| JP2001194307A | ||||
| JP11064279A | ||||
| JP11326171A | ||||
| JP6027122A |
Foreign References:
| GB967586A | ||||
| DE19736641A1 |
Attorney, Agent or Firm:
Sonoda Yoshitaka
Kobayashi Yoshinori
Kobayashi Yoshinori