Title:
催涙成分生成酵素活性を有する蛋白質又はポリペプチド、該蛋白質又はポリペプチドをコードするDNA、該DNAを用いた催涙成分生成酵素活性を有する蛋白質又はポリペプチドの製造方法及び該蛋白質又はポリペプチドについてのmRNAの翻訳を阻害する機能を有する核酸分子
Document Type and Number:
Japanese Patent JP4611638
Kind Code:
B2
Abstract:
A DNA encoding a protein or polypeptide with an effect of converting 1-propenylsulfenic acid into a lachrymator, is new. Independent claims are also included for: (1) a primer for isolating the lachrymator synthase gene of a plant belonging to genus Allium, which contains a sequence of (XXV) comprising 26 base pairs; (2) isolating the Allium lachrymator synthase gene by using the primer; (3) a recombinant vector containing any of the DNAs; (4) a transformant containing the recombinant vector; (5) producing the protein or polypeptide with lachrymator synthase activity; (6) a protein or polypeptide with lachrymator synthase activity containing: (a) an amino acid sequence of (II), (VI), (VIII), (X), (XIV) or (XVI) comprising 164-169 amino acids; (b) an amino acid sequence based on the sequence of (II), (VI), (VIII), (X), (XIV) or (XVI) but with some amino acids added, deleted or substituted; or (c) an amino acid sequence comprising 65 % homology with the sequence of (II), (VI), (VIII), (X), (XIV) or (XVI); and (7) a nucleic acid molecule with a function of inhibiting the translation of mRNA concerning the protein or polypeptide. ACTIVITY : Ophthalmological. No biological data given. MECHANISM OF ACTION : None given.
Inventors:
Shinsuke Imai
Nobuaki Tsuge
Toshihiro Kamada
Noriya Masamura
Masano
Nobuaki Tsuge
Toshihiro Kamada
Noriya Masamura
Masano
Application Number:
JP2003573155A
Publication Date:
January 12, 2011
Filing Date:
February 28, 2003
Export Citation:
Assignee:
House Foods Co., Ltd.
International Classes:
C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/90; C12N15/09; C12N15/29; C12N15/82
Domestic Patent References:
| JP10295373A |
Other References:
Biosci.Biotech.Biochem., vol.61(2), pp.251-255 (1997)
Biosci.Biotech.Biochem., vol.63(4), pp.726-730 (1999)
Biochem.J., vol.283, pp.217-222 (1992)
Biosci.Biotech.Biochem., vol.63(4), pp.726-730 (1999)
Biochem.J., vol.283, pp.217-222 (1992)
Attorney, Agent or Firm:
Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama
Kazuo Yamazaki
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama
Kazuo Yamazaki