Title:
自然放出蛍光をパルス励起、連続脱励起、およびゲート記録するＳＴＥＤ顕微鏡法、ＳＴＥＤ蛍光相関分光法、およびＳＴＥＤ蛍光顕微鏡
Document Type and Number:
Japanese Patent JP5776992
Kind Code:
B2
Abstract:
In a STED fluorescence light microscope pulses of excitation light (3) are applied to a sample, which excite fluorescent entities contained in the sample for fluorescence, and which are focused on at least one focal area. Further, de-excitation light (12) is applied to the sample, which de-excites the excited fluorescent entities and which comprises an intensity zero point in the at least one focal area, as a continuous wave. Fluorescence light emitted by the excited fluorescent entities in the sample is registered after each pulse of the excitation light (3) and overlapping with applying the de-excitation light (13) with high temporal resolution between consecutive pulses of the excitation light (3).
Inventors:
Hell, Stephen W.
Engelhard, Johann
Reus, Matthias
West Fal, Volker
Eggering, Christian
Moneron, gel
Han, Kyu Young
Bisidmini, Giuseppe
Katrin, Willig
Engelhard, Johann
Reus, Matthias
West Fal, Volker
Eggering, Christian
Moneron, gel
Han, Kyu Young
Bisidmini, Giuseppe
Katrin, Willig
Application Number:
JP2013539143A
Publication Date:
September 09, 2015
Filing Date:
November 22, 2010
Export Citation:
Assignee:
Max-Plank-Gezelle Shaft Crane Forderung Del Wiszenshakhten E.V.
Deutches Krebs Forsung Zentrum
Deutches Krebs Forsung Zentrum
International Classes:
G01N21/64
Domestic Patent References:
| JP11095120A | ||||
| JP2000241782A | ||||
| JP2010537179A | ||||
| JP2004077175A |
Foreign References:
| WO2009024529A1 |
Other References:
Auksoriua E , et.al.,"Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging",OPTICS LETTERS,2008年 1月15日,Vol.33, No.2,p.113-115
Attorney, Agent or Firm:
▲吉▼川 俊雄