Title:
標的塩基配列の識別方法
Document Type and Number:
Japanese Patent JP5843112
Kind Code:
B2
Abstract:
According to the present invention, a method for identifying a base sequence accompanying competitive hybridization is provided, wherein a nucleic acid having a target base sequence can be identified in an extremely short period of time as compared with conventional methods while maintaining the accuracy of identifying differences in base sequences. The method of the present invention comprises a thermal denaturation step for subjecting a sample double-stranded nucleic acid and a reference double-stranded nucleic acid containing the same base sequence as a target base sequence to thermal denaturation treatment in a single reaction solution, a temperature lowering step for carrying out competitive hybridization between the sample double-stranded nucleic acid and the reference double-stranded nucleic acid by lowering the temperature of the reaction solution after the thermal denaturation step, a measurement step for measuring a double-stranded nucleic acid formed by a nucleic acid strand that composed the reference double-stranded nucleic acid and a nucleic acid strand that composed the sample double-stranded nucleic acid, and an identification step for identifying identity between the reference double-stranded nucleic acid and the sample double-stranded nucleic acid based on measurement results obtained from the measurement step; wherein, the temperature lowering step is carried out in the presence of a cationic comb-type polymer.
Inventors:
Kitano Shiro
Akio Yamane
Maruyama Atsushi
Akio Yamane
Maruyama Atsushi
Application Number:
JP2012508268A
Publication Date:
January 13, 2016
Filing Date:
March 25, 2011
Export Citation:
Assignee:
Toppan Printing Co., Ltd.
Kyushu University
Kyushu University
International Classes:
C12Q1/68; C12N15/09
Foreign References:
| WO1995002068A1 | 1995-01-19 | |||
| WO2003018841A1 | 2003-03-06 |
Other References:
JPN6015011165; 'Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｓｙｍｐｏｓｉｕｍ Ｓｅｒｉｅｓ' ２００８， Ｖｏｌ．５２， ｐｐ．７１５-７１６
JPN6011033483; ＫＩＭ ＷＪ． ｅｔ ａｌ．，: 'Ｃａｔｉｏｎｉｃ ｃｏｍｂ-ｔｙｐｅ ｃｏｐｏｌｙｍｅｒｓ ｆｏｒ ＤＮＡ ａｎａｌｙｓｉｓ．' Ｎａｔｕｒｅ Ｍａｔｅｒｉａｌｓ， ２００３， Ｖｏｌ．２， ｐｐ．８１５-８２０
JPN6011033468; ＷＯ Ｌ． ｅｔ ａｌ．，: 'Ｅｆｆｅｃｔ ｏｆ ｐｏｌｙ（Ｌ-ｌｙｓｉｎｅ）-ｇ-ｄｅｘｔｒａｎ ｃｏｐｏｌｙｍｅｒｓ ｏｎ ＤＮＡ ｈｙｂｒｉｄｉｚａｔｉｏｎ．' Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｓｙｍｐｏｓｉｕｍ Ｓｅｒｉｅｓ， ２００７， Ｎｏ．５１， ｐｐ．７３-７４
JPN6015011166; 'Ｃｈｅｍｉｓｔｒｙ Ａ Ｅｕｒｏｐｅａｎ Ｊｏｕｒｎａｌ' ２００１， Ｖｏｌ．７， Ｎｏ．１， ｐｐ．１７６-１８０
JPN6011033462; ＭＡＥＤＡ Ｓ． ｅｔ ａｌ．，: 'Ｄｅｔｅｃｔｉｏｎ ｏｆ Ｃｌａｒｉｔｈｒｏｍｙｃｉｎ-Ｒｅｓｉｓｔａｎｔ Ｈｅｌｉｃｏｂａｃｔｅｒ ｐｙｌｏｒｉ Ｓｔｒａｉｎｓ ｂｙ ａ Ｐｒｅｆｅｒｅｎｔｉａｌ Ｈｏｍｏｄｕｐｌｅｘ Ｆｏｒｍａ' Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｍｉｃｒｏｂｉｏｌｏｇｙ， ２０００， Ｖｏｌ．３８， Ｎｏ．１， ｐｐ．２１０-２１４
JPN6011033464; 丸山 厚: '生体材料と核酸技術．' バイオマテリアル-生体材料， ２００８， Ｖｏｌ．２６， Ｎｏ．６， ｐｐ．４３３-４４０
JPN6011033467; 丸山 厚: '分子シャペロン工学に基づく遺伝子解析．' 独立行政法人科学技術振興機構（ＪＳＴ）戦略的創造研究推進事業（ＣＲＥＳＴ）第４回公開シンポジウム テ
JPN6011033487; ＷＵ Ｌ． ｅｔ ａｌ．，: 'Ｐｏｌｙ（Ｌ-ｌｙｓｉｎｅ）-ｇｒａｆｔ-ｄｅｘｔｒａｎｃｏｐｏｌｙｍｅｒ ａｃｃｅｌｅｒａｔｅｓ ＤＮＡ ｈｙｂｒｉｄｉｚａｔｉｏｎ ｂｙ ｔｗｏ ｏｒｄｅｒｓ．' Ｓｏｆｔ Ｍａｔｔｅｒ， ２００８， Ｖｏｌ．４， ｐｐ．７４４-７４７
JPN6015011161; 'Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ' ２００８， Ｖｏｌ．３６， Ｎｏ．１， ｐｐ．３４２-３５１
JPN6015011163; 'Ｊｏｕｒｎａｌ ｏｆ Ａｍｅｒｉｃａｎ Ｃｈｅｍｉｃａｌ Ｓｏｃｉｅｔｙ' ２００２， Ｖｏｌ．１２４， ｐｐ．１２６７６-１２６７７
JPN6011033485; ＳＨＩＭＡＤＡ Ｎ． ｅｔ ａｌ．，: 'Ｎｏｖｅｌ ａｎａｌｙｓｉｓ ｆｏｒ ｓｉｎｇｌｅ ｎｕｃｌｅｏｔｉｄｅ ｐｏｌｙｍｏｒｐｈｉｓｍ ｕｓｉｎｇ ｃａｔｉｏｎｉｃ ｃｏｍｂ-ｔｙｐｅ ｃｏｐｏｌｙｍｅｒｓ．' Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｓｙｍｐｏｓｉｕｍ Ｓｅｒｉｅｓ， ２００７， Ｎｏ．５１， ｐｐ．３３９-３４０
JPN6011033483; ＫＩＭ ＷＪ． ｅｔ ａｌ．，: 'Ｃａｔｉｏｎｉｃ ｃｏｍｂ-ｔｙｐｅ ｃｏｐｏｌｙｍｅｒｓ ｆｏｒ ＤＮＡ ａｎａｌｙｓｉｓ．' Ｎａｔｕｒｅ Ｍａｔｅｒｉａｌｓ， ２００３， Ｖｏｌ．２， ｐｐ．８１５-８２０
JPN6011033468; ＷＯ Ｌ． ｅｔ ａｌ．，: 'Ｅｆｆｅｃｔ ｏｆ ｐｏｌｙ（Ｌ-ｌｙｓｉｎｅ）-ｇ-ｄｅｘｔｒａｎ ｃｏｐｏｌｙｍｅｒｓ ｏｎ ＤＮＡ ｈｙｂｒｉｄｉｚａｔｉｏｎ．' Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｓｙｍｐｏｓｉｕｍ Ｓｅｒｉｅｓ， ２００７， Ｎｏ．５１， ｐｐ．７３-７４
JPN6015011166; 'Ｃｈｅｍｉｓｔｒｙ Ａ Ｅｕｒｏｐｅａｎ Ｊｏｕｒｎａｌ' ２００１， Ｖｏｌ．７， Ｎｏ．１， ｐｐ．１７６-１８０
JPN6011033462; ＭＡＥＤＡ Ｓ． ｅｔ ａｌ．，: 'Ｄｅｔｅｃｔｉｏｎ ｏｆ Ｃｌａｒｉｔｈｒｏｍｙｃｉｎ-Ｒｅｓｉｓｔａｎｔ Ｈｅｌｉｃｏｂａｃｔｅｒ ｐｙｌｏｒｉ Ｓｔｒａｉｎｓ ｂｙ ａ Ｐｒｅｆｅｒｅｎｔｉａｌ Ｈｏｍｏｄｕｐｌｅｘ Ｆｏｒｍａ' Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｍｉｃｒｏｂｉｏｌｏｇｙ， ２０００， Ｖｏｌ．３８， Ｎｏ．１， ｐｐ．２１０-２１４
JPN6011033464; 丸山 厚: '生体材料と核酸技術．' バイオマテリアル-生体材料， ２００８， Ｖｏｌ．２６， Ｎｏ．６， ｐｐ．４３３-４４０
JPN6011033467; 丸山 厚: '分子シャペロン工学に基づく遺伝子解析．' 独立行政法人科学技術振興機構（ＪＳＴ）戦略的創造研究推進事業（ＣＲＥＳＴ）第４回公開シンポジウム テ
JPN6011033487; ＷＵ Ｌ． ｅｔ ａｌ．，: 'Ｐｏｌｙ（Ｌ-ｌｙｓｉｎｅ）-ｇｒａｆｔ-ｄｅｘｔｒａｎｃｏｐｏｌｙｍｅｒ ａｃｃｅｌｅｒａｔｅｓ ＤＮＡ ｈｙｂｒｉｄｉｚａｔｉｏｎ ｂｙ ｔｗｏ ｏｒｄｅｒｓ．' Ｓｏｆｔ Ｍａｔｔｅｒ， ２００８， Ｖｏｌ．４， ｐｐ．７４４-７４７
JPN6015011161; 'Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ' ２００８， Ｖｏｌ．３６， Ｎｏ．１， ｐｐ．３４２-３５１
JPN6015011163; 'Ｊｏｕｒｎａｌ ｏｆ Ａｍｅｒｉｃａｎ Ｃｈｅｍｉｃａｌ Ｓｏｃｉｅｔｙ' ２００２， Ｖｏｌ．１２４， ｐｐ．１２６７６-１２６７７
JPN6011033485; ＳＨＩＭＡＤＡ Ｎ． ｅｔ ａｌ．，: 'Ｎｏｖｅｌ ａｎａｌｙｓｉｓ ｆｏｒ ｓｉｎｇｌｅ ｎｕｃｌｅｏｔｉｄｅ ｐｏｌｙｍｏｒｐｈｉｓｍ ｕｓｉｎｇ ｃａｔｉｏｎｉｃ ｃｏｍｂ-ｔｙｐｅ ｃｏｐｏｌｙｍｅｒｓ．' Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｓｙｍｐｏｓｉｕｍ Ｓｅｒｉｅｓ， ２００７， Ｎｏ．５１， ｐｐ．３３９-３４０
Attorney, Agent or Firm:
Shiro Suzuki
Masatake Shiga
Tadashi Takahashi
Shunsuke Fushimi
Masatake Shiga
Tadashi Takahashi
Shunsuke Fushimi