Title:
抗血清アルブミンFABエフェクター部分融合コンストラクト、およびその製造方法
Document Type and Number:
Japanese Patent JP6422977
Kind Code:
B2
Abstract:
The present invention relates to antigen-binding fragment(Fab) and a Fab-effector fusion protein or (poly)peptide comprising thereof. The Fab of the present invention specifically binds to serum albumin and thereby has extendedin vivohalf-life. The Fab of the present invention is characterized by not having cysteine residues that are responsible for the interchain disulfide bond in CH1domain and CkLdomain as well. The Fab-effector fusion protein or (poly)peptide of the present invention can be produced in periplasm ofE. coliwith high yield, and has increasedin vivohalf-life. Further, the present invention providesE. colistrain which produces various kinds of Fab-effector fusion proteins or (poly)peptides, and a pharmaceutical composition comprising the fab-effector fusion proteins or (poly)peptides.
Inventors:
Cha, San Hun
Application Number:
JP2016538860A
Publication Date:
November 14, 2018
Filing Date:
August 29, 2014
Export Citation:
Assignee:
April Bio Company Limited
International Classes:
C07K16/18; C07K19/00; C12N1/21; C12N15/13; C12N15/62; C12N15/63
Domestic Patent References:
| JP2007535472A | ||||
| JP2008500830A | ||||
| JP2010518062A | ||||
| JP2010539921A | ||||
| JP2013505923A | ||||
| JP2012510803A | ||||
| JP2013506628A | ||||
| JP2006512895A |
Foreign References:
| WO2013068571A1 | ||||
| WO2006085518A1 |
Other References:
GRIFFIN, L. M. et al.,"Analysis of heavy and light chain sequences of conventional camelid antibodies from Camelus dromedarius and Camelus bactrianus species",J. Immunol. Methods,2014年 1月18日,Vol. 405,pp. 35-46
Attorney, Agent or Firm:
Kiyoji Kuzuwa