Title:
肝細胞系譜細胞を取得するための方法および物質
Document Type and Number:
Japanese Patent JP6792857
Kind Code:
B2
Abstract:
Methods of producing hepatocyte lineage cells are provided. The method can include transfecting a cell with one or more expression vectors. For example, a cell can be transfected with a first expression vector containing a first gene that encodes CCAAT/enhancer binding protein alpha (CEBPA), a second expression vector containing a second gene that encodes CCAAT/enhancer binding protein beta (CEBPB), a third expression vector containing a third gene that encodes forkhead box A1 (FOXA1), and a fourth expression vector containing a fourth gene that encodes forkhead box A3 (FOXA3). The method can include culturing the transfected cell obtained in a growth environment. The transfected cell can be cultured in Williams' E medium, ReproFF (feeder-free media maintaining pluripotency) medium, or both. Transfected and/or hepatocyte lineage cells obtained by a method of the present invention are also provided.
Inventors:
Minoru Tomizawa
Application Number:
JP2016138602A
Publication Date:
December 02, 2020
Filing Date:
July 13, 2016
Export Citation:
Assignee:
National University Corporation Chiba University
International Classes:
C12N5/10; C12N15/12
Domestic Patent References:
| JP2013523183A |
Foreign References:
| WO2011052504A1 |
Attorney, Agent or Firm:
Takashi Shoji
Yuriko Shinobu
Takuya Osugi
Aki Soga
Yuriko Shinobu
Takuya Osugi
Aki Soga