Title:
核酸検出方法及びアッセイキット
Document Type and Number:
Japanese Patent JP6862502
Kind Code:
B2
Abstract:
To provide a nucleic acid detection method with which nucleic acids can be detected conveniently at high sensitivity and an assay kit.SOLUTION: An embodiment of a nucleic acid detection method is for detecting a target nucleic acid in a sample in which the target nucleic acid includes a first sequence. The method comprises: (A) putting, in the presence of an electrode, a reaction liquid that comprises the sample, a primer set, amplification enzyme, magnesium ions at a concentration of 4 mM to 30 mM, and a ruthenium complex redox probe that generates a detectable electrical signal that increases in strength as the amount of amplification product increases in the reaction field and forming a reaction field in which the nucleic acid probe is not immobilized; (B) keeping the reaction field under amplification reaction conditions; (C) detecting electrical signals from the redox probe over time while keeping the reaction field under amplification reaction conditions; and (D) determining the existence or amount of the target nucleic acid on the basis of the strength of the electrical signal over time obtained in (C).SELECTED DRAWING: Figure 1
Inventors:
Koji Hashimoto
Keiko Ito
Inasa Mimasa
Keiko Ito
Inasa Mimasa
Application Number:
JP2019130982A
Publication Date:
April 21, 2021
Filing Date:
July 16, 2019
Export Citation:
Assignee:
Toshiba Corporation
International Classes:
C12Q1/6876; C12M1/00; C12N15/09; C12Q1/686; G01N33/483; G01N33/50
Domestic Patent References:
| JP2010154834A | ||||
| JP2007295811A | ||||
| JP2010098963A | ||||
| JP2009501532A |
Foreign References:
| WO2016136033A1 | ||||
| WO2005056790A1 | ||||
| WO2004011643A1 |
Other References:
Analyst,2013年,Vol.138,p.907-915
Attorney, Agent or Firm:
Suzue International Patent Office