Title:
ＭＡＲＣＫＳのリン酸化を指標にしたＡＤ（アルツハイマー病）、ＦＴＬＤ（前頭側頭葉変性症）、ＡＬＳ（筋萎縮性側索硬化症）、ＰＤ（パーキンソン病）およびＤＬＢ（レビー小体型認知症）の検出
Document Type and Number:
Japanese Patent JP7329497
Kind Code:
B2
Abstract:
Provided is a method for detecting a neurodegenerative disease selected from the group consisting of human Alzheimer's disease (AD), frontotemporal lobar degeneration (FTLD), and amyotrophic lateral sclerosis (ALS) with high sensitivity and high specificity. A method for detecting a neurodegenerative disease selected from the group consisting of human Alzheimer's disease (AD), frontotemporal lobar degeneration (FTLD), and amyotrophic lateral sclerosis (ALS), comprising the steps of: (i) measuring a MARCKS protein phosphorylated at position 46 and a non-phosphorylated MARCKS protein in a test specimen collected from a subject; (ii) calculating a DO value expressed by the following formula from the measured values obtained in (i): DO=pSer46-MARCKS2+non-phosphorylated-MARCKS2 where "pSer46-MARCKS" represents the amount of a MARCKS protein phosphorylated at position 46; and "non-phosphorylated-MARCKS" represents the amount of a non-phosphorylated MARCKS protein; and (iii) detecting a neurodegenerative disease based on the DO value as an indicator.
Inventors:
Hitoshi Okazawa
Application Number:
JP2020504057A
Publication Date:
August 18, 2023
Filing Date:
March 08, 2019
Export Citation:
Assignee:
National University Corporation Tokyo Medical and Dental University
Denka Company Limited
Denka Company Limited
International Classes:
G01N33/68; G01N27/62
Foreign References:
| WO2015099094A1 | ||||
| US20130196925 |
Other References:
FUJITA, K. et al.,HMGB1, a pathogenic molecule that induces neurite degeneration via TLR4-MARCKS, is a potential therapeutic target for Alzheimer's disease,SCIENTIFIC REPORTS,2016年08月25日,Vol.6/No.31895,pp.1-15,DOI:10.1038/srep31895
TAGAWA, K.,Comprehensive phosphoproteome analysis unravels the core signaling network that initiates the earliest synapse pathology in preclinical Alzheimer's disease brain,HUMAN MOLECULAR GENETICS,2014年09月17日,Vol.24/No.2,pp.540-558,DOI:10.1093/hmg/ddu475
Kimura T et al.,Phosphorylation of MARCKS in Alzheimer disease brains,Neuroreport,2000年03月20日,Vol. 11, No. 4,pp.869-873
TAGAWA, K.,Comprehensive phosphoproteome analysis unravels the core signaling network that initiates the earliest synapse pathology in preclinical Alzheimer's disease brain,HUMAN MOLECULAR GENETICS,2014年09月17日,Vol.24/No.2,pp.540-558,DOI:10.1093/hmg/ddu475
Kimura T et al.,Phosphorylation of MARCKS in Alzheimer disease brains,Neuroreport,2000年03月20日,Vol. 11, No. 4,pp.869-873
Attorney, Agent or Firm:
Patent Attorney Corporation Hiraki International Patent Office