Title:
【発明の名称】LAK細胞の産生増殖方法
Document Type and Number:
Japanese Patent JPH05505093
Kind Code:
A
Abstract:
Process for generating and expanding cells having LAK cell activity comprises (a) treating human PBMC to deplete monocytes, (b) culturing the resulting peripheral blood lymphocytes in medium containing IL-2 and human plasma or serum to generate LAK activity and (c) expanding the cells by culturing at low cell density in medium containing IL-2 and human plasma or serum in a culture bag made from and organic polymeric, oxygen permeable film. Monocytes are depleted by treating the PBMC with and ester of an L-amino acid such as phenylalanine methyl ester.
Inventors:
Ill, Joseph. David
Reinhardt, John Joseph
Reinhardt, John Joseph
Application Number:
JP50711090A
Publication Date:
August 05, 1993
Filing Date:
April 30, 1990
Export Citation:
Assignee:
Terumo Corporation
International Classes:
A61K35/14; A61P37/00; C08F10/00; C08F210/00; C08F210/02; C12N5/02; C12N5/0783; C12R1/91; (IPC1-7): A61K35/14; C08F210/02; C12N5/02; C12N5/08
Attorney, Agent or Firm:
Ryoko Tsuji