【発明の名称】マンノースまたはキシロースに基づく陽性選択

Japanese Patent JPH08509861

A

PCT No. PCT/EP94/00575 Sec. 371 Date Oct. 3, 1995 Sec. 102(e) Date Oct. 3, 1995 PCT Filed Feb. 28, 1994 PCT Pub. No. WO94/20627 PCT Pub. Date Sep. 15, 1994The present invention provides a method for identifying or selecting from a population of eukaryotic cells cultivated on or in a medium containing at least one compound, cells which have a metabolic advantage as a result of having being transformed, wherein: i) the cells are transformed with a nucleotide sequence or a co-introduced nucleotide sequence one of which comprises a region which: (a) encodes a protein which is involved in the metabolism of the compound, and/or (b) regulates the activity of the protein; and ii) the compound is mannose or xylose or a derivative or a precursor of these, or a substrate of the protein, or is capable of being metabolized by the transformed cells into such a substrate, with the proviso that the compound is not mannose when the protein is mannose 6 phosphate isomerase. The invention also includes a method according to the preceding paragraph wherein the compounds are not so limited with the proviso that an agent which reduces the toxicity to the cells of the compound is added to the medium. It is preferred that where a toxicity-reducing agent has been added to the culture medium, the compound is mannose and the nucleotide or co-introduced nucleotide sequence encodes mannose-6-phosphate isomerase.

Boysen, Kiasten
Donaldson, Earin
Hall Rup, Anna
Jearsbo, Morten
Clay Beau, Jette Dina
Nielsen, Yoon
Okers, Fin Teige
Petersen, Steen Gruyer

JP51953794A

October 22, 1996

February 28, 1994

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Sand Limited

A01H5/00; C07H15/203; C07H15/26; C07H17/02; C07H19/16; C07H19/20; C12N5/00; C12N5/10; C12N9/24; C12N9/90; C12N9/92; C12N15/09; C12N15/29; C12N15/65; C12N15/82; C12Q1/02; (IPC1-7): C12N15/09; A01H5/00; C12N5/10; C12N9/90

Aoyama Ryo (1 person outside)

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