Title:
【発明の名称】電気化学発光性酵素バイオセンサー
Document Type and Number:
Japanese Patent JPH11507536
Kind Code:
A
Abstract:
Electrochemiluminescent enzymes, their preparation and use as biosensors are disclosed. Specifically, two appendages are covalently attached to a desired dehydrogenase enzyme; (1) a nicotinamide adenine cofactor or analog thereof, and (2) a luminescent ruthenium complex. For example, glucose concentrations is the following way. A doubly-modified glucose dehydrogenase could oxidize glucose with concomitant reduction of the attached NAD<+> to NADH. Because NADH, but not NAD<+>, is able to interact with surface ruthenium to promote ECL, only enzyme molecules that have reacted with glucose will emit light from their ruthenium label in an ECL instrument. The relative close proximity of NADH and ruthenium on the enzyme surface enhances light emission as compared to the same concentrations in free solution. When NADH reduces ruthenium, it returns to become NAD<+>, permitting multiple cycles of ECL light emission from a single enzyme molecule. Such biosensors can be used in solution or bound to a solid surface. Assays employing the biosensor molecules can be performed on an IGEN OrigenAnalyzer.
Inventors:
Martin, Mark Tee.
Application Number:
JP50205997A
Publication Date:
July 06, 1999
Filing Date:
June 06, 1996
Export Citation:
Assignee:
Igen, Inc.
International Classes:
C12Q1/00; G01N21/66; G01N21/76; G01N21/78; C12Q1/32; G01N27/327; G01N27/42; G01N33/535; (IPC1-7): C12Q1/32; G01N21/78; G01N27/327; G01N27/42
Attorney, Agent or Firm:
Akira Asamura (3 outside)