To obtain the subject new cosmetic by compounding a phosphorylated polysaccharide having humectant action and collagen-producing performance as an active component.
This cosmetic contains a compound of the formula (Glc is glucose residue; Gal is galactose residue; Rha is rhamnose residue; (m) is 0-3; (n) is 1,000-5,000) as an active component. The component of the formula can be produced by hydrolyzing actinase E of defatted milk, treating the defatted milk with ultrafiltration membrane, sterilizing the filtrate to obtain a culture medium, aseptically pipetting the sterilized medium into a jar fermenter, inoculating a precultured liquid, culturing at 20°C and pH5.5 using NH3 water as a neutralizing agent, subjecting the cultured product to centrifugal separation after culture to recover the supernatant, adding an equal amount of CH3OH, recovering the produced precipitate, dissolving the precipitate in 0.2N saline water, repeating the precipitation operation with CH3OH, subjecting the treated precipitate to electrophoresis, recovering the component impregnated into the gel and purifying the recovered fraction by ion-exchange chromatography to obtain purified polysaccharide. It is necessary to compound a cosmetic with ≥0.001wt.% of the compound of the formula.
OBA TETSUO
TANIMOTO MORIMASA
OTA YOSHIYUKI
KLAUS DOESBURG
JAN SIKKEMA
IWASAKI TAISUKE
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