Title:
試料中において任意には分画された後の試料中において、固定化特異的結合パートナーとして測定される分析対象物を用いる分析プラットフォーム及び検出法
Document Type and Number:
Japanese Patent JP2005537487
Kind Code:
A
Abstract:
The present invention is related to an analytical platform and a method performed therewith for the analysis of multiple samples for analytes which are contained therein and are of biological relevance as binding partners in specific binding reactions, wherein said samples or fractions of said samples, with the analytes to be determined contained therein, as a first plurality of specific binding partners, are deposited directly or after additional dilutions of said samples or fractions in discrete measurement areas in at least one one- or two-dimensional array of measurement areas on an evanescent field sensor platform as a solid support, different samples or fractions or different dilutions of samples or fractions being arranged in different discrete measurement areas, one or more tracer compounds as a second plurality of specific binding partners, for the specific determination of one or more analytes out of the first plurality of specific binding partners contained in the samples, are brought into contact with the samples or their fractions or dilutions deposited in said discrete measurement areas in a single step or multiple steps of a specific binding reaction, changes in opto-electronic signals, resulting from the binding of tracer compounds to analytes contained in discrete measurement areas in the evanescent field of the evanescent field sensor platform are measured laterally resolved, and the presence of the analytes to be specifically detected is determined qualititatively and/or quantitatively from the relative amount of the changes in said opto-electronic signals from the corresponding measurement areas.
Inventors:
Paul, Michael
Chic, Egginhard
Orochelan, Peter
Chic, Egginhard
Orochelan, Peter
Application Number:
JP2004533419A
Publication Date:
December 08, 2005
Filing Date:
August 28, 2003
Export Citation:
Assignee:
Zeptosens AG
International Classes:
C12M1/00; C12M1/34; C12Q1/02; C12Q1/68; G01N21/25; G01N21/55; G01N21/64; G01N21/76; G01N21/78; G01N27/447; G01N30/88; G01N33/543; G01N33/53; G01N33/553; G01N33/577; (IPC1-7): G01N33/53; C12M1/00; C12M1/34; C12Q1/02; C12Q1/68; G01N21/76; G01N21/78; G01N27/447; G01N30/88; G01N33/543; G01N33/553; G01N33/577
Domestic Patent References:
| JP2003501654A | 2003-01-14 | |||
| JP2002065299A | 2002-03-05 |
Attorney, Agent or Firm:
Hajime Tsukuni
Fumio Shinoda
Fumio Shinoda