Title:
抗体の製造方法
Document Type and Number:
Japanese Patent JP4794301
Kind Code:
B2
Abstract:
Focusing on the fact that antibody molecules with one H chain are not secreted when using the "knobs-into-holes" method, the present inventors revealed that desired bispecific antibodies can be preferentially formed by first expressing the H and L chains of one arm, and then suppressing their expression, followed by expressing the H and L chains of the other arm so that first desired HL molecules (HaLa and HbLb) are constructed, and then the H chains are paired with each other (H2L2). The present invention was thus completed.
Inventors:
Taro Miyazaki
Tetsuro Kojima
Tetsuro Kojima
Application Number:
JP2005507008A
Publication Date:
October 19, 2011
Filing Date:
June 11, 2004
Export Citation:
Assignee:
Chugai Pharmaceutical Co., Ltd.
International Classes:
C12N15/09; C07K16/00; C07K16/46; C12N1/15; C12N1/19; C12N1/21; C12P21/08
Other References:
J. Immunol. Methods, (2001), 248, [1-2], p.7-15
Protein Eng., (1996), 9, [7], p.617-621
Biochem. Soc. Trans., (2002), 30, [4], p.507-511
J. Exp. Med., (1992), 175, [1], p.217-225
Gene, (1994), 151, [1-2], p.131-135
Protein Eng., (1996), 9, [7], p.617-621
Biochem. Soc. Trans., (2002), 30, [4], p.507-511
J. Exp. Med., (1992), 175, [1], p.217-225
Gene, (1994), 151, [1-2], p.131-135
Attorney, Agent or Firm:
Hatsushi Shimizu
Koichi Niimi
Koichi Niimi