Title:
CELL DETECTION METHOD AND KIT
Document Type and Number:
Japanese Patent JP2021185917
Kind Code:
A
Abstract:
To provide a cell detection method capable of detecting a target cell in a biological specimen, with a simplified system.SOLUTION: A cell detection method comprises: a step for adding a nucleic acid aptamer with a tag which is formed of a single chain nucleic acid, to a solution including a target cell, and specifically coupling the nucleic acid aptamer to a cell surface substance of the target cell; a step for adding a trigger nucleic acid complex including a trigger nucleic acid comprising a partial base sequence of a tag part in the nucleic acid aptamer with a tag, and a mask nucleic acid including a base sequence which is complementary to the base sequence of the tag part, for discharging the trigger nucleic acid; a step for adding a cast nucleic acid complex in which, both modification potions are provided in proximate positions for suppressing signal, in a tandem double chain formed by a nucleic acid A and a nucleic acid B in which, a signal emission molecule and a signal suppression molecule are modified, and a cast nucleic acid, and a fuel nucleic acid, to the solution including the discharged trigger nucleic acid, and rotating a nucleic acid circuit, for amplifying the signal; and a step for detecting the amplified signal.SELECTED DRAWING: Figure 1
Inventors:
KITAMURA YUSUKE
IHARA TOSHIHIRO
NAKAJIMA YUTA
KATSUTA YOSUKE
IHARA TOSHIHIRO
NAKAJIMA YUTA
KATSUTA YOSUKE
Application Number:
JP2021090562A
Publication Date:
December 13, 2021
Filing Date:
May 28, 2021
Export Citation:
Assignee:
UNIV KUMAMOTO
International Classes:
C12Q1/04; C12Q1/6818
Attorney, Agent or Firm:
Yasuo Hirai