Title:
COMPOSITION FOR CLEAVING TARGET DNA COMPRISING GUIDE RNA SPECIFIC FOR TARGET DNA AND CAS PROTEIN-ENCODING NUCLEIC ACID OR CAS PROTEIN, AND USE THEREOF
Document Type and Number:
Japanese Patent JP2023166401
Kind Code:
A
Abstract:
To provide a composition and a method for inducing the targeted disruption of endogenous genes within eukaryotic cells.SOLUTION: The present invention provides a composition for inducing the targeted disruption of endogenous genes within eukaryotic cells, including a Cas9/RNA complex. The Cas9/RNA complex includes a Cas9 protein bound to one or more tag sequences, wherein the tag sequence includes a nuclear localization signal (NLS)) and a guide RNA having CRISPR RNA (crRNA) and trans-activating crRNA (tracrRNA), wherein crRNA includes (i) a part that hybridizes with a part of tracrRNA and ii) a part complementary to the target DNA of the endogenous gene. Prior to its introduction into eukaryotic cells, the Cas9 protein is complexed with the guide RNA to form a ribonucleoprotein. The ribonucleoprotein serves as an endonuclease that induces the targeted disruption of the target DNA.SELECTED DRAWING: Figure 1a
Inventors:
KIM JIN-SOO
CHO SEUNG WOO
KIM SO JUNG
KIM JONG MIN
KIM SEOKJOONG
CHO SEUNG WOO
KIM SO JUNG
KIM JONG MIN
KIM SEOKJOONG
Application Number:
JP2023131350A
Publication Date:
November 21, 2023
Filing Date:
August 10, 2023
Export Citation:
Assignee:
TOOLGEN INC
International Classes:
C12N15/09; C12N9/22; C12N15/11; C12N15/52; C12N15/79; C12P21/02
Attorney, Agent or Firm:
Shinichiro Tanaka
▲吉▼田 和彦
Hiroyuki Suda
Kazuo Yamazaki
Satsuki Ichikawa
Hironobu Hattori
Nanae Matsuda
Gen Tashiro
▲吉▼田 和彦
Hiroyuki Suda
Kazuo Yamazaki
Satsuki Ichikawa
Hironobu Hattori
Nanae Matsuda
Gen Tashiro