CYCLIC DOUBLE-STRANDED DNA, MICROORGANISM CONTAINING SAID DNA AND PRODUCTION OF POLIOVIRUS CAPSID PROTEIN P1 USING SAME

Japanese Patent JPS62215390

A

PURPOSE: A bacterium in Escherichiae transformed with a plasma containing a specific cyclic double-stranded DNA is cultured to collect the fused protein accumulated whereby poliovirus capsid protein P1, which is used as a vaccine, is obtained.

CONSTITUTION: A plasmid containing 3 DNA regions coding (1) high manifestation promoter operator and the amino-terminal moiety in the m-pyrocatechol gene containing the SD sequence of the pyrocatechol gene which follows the operator and is affected by it, (2) the DNA part which follows the above region and coding the capsid protein of attenuated poliovirus Sabin 1, 3 or 3 strain and (3) a part coding the anpicillin resistance is prepared. A bacterium in Escherichia is transformed with the plasmid and cultured in a nutrient medium. Then, the fused protein accumulated in the cell bodies is collected to obtain the objective poliovirus capsid protein P1.

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TAKAHARA YOSHIYUKI
OMORI MUNEKI
NUMAO OSANORI

JP5806586A

September 22, 1987

March 18, 1986

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SAGAMI CHEM RES
CENTRAL GLASS CO LTD
HODOGAYA CHEMICAL CO LTD
NIPPON SODA CO
NISSAN CHEMICAL IND LTD
TOYO SODA MFG CO LTD

C12N15/09; A61K39/13; C07K14/105; C12N1/20; C12N15/70; C12R1/19; (IPC1-7): A61K39/13; C07H21/04; C12N1/20; C12N15/00; C12P21/02; C12R1/19

Hajime Tsukuni

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