PURPOSE: To enable production of edible fats and oils having excellent emulsifying characteristics without adding an emulsifying agent thereto by carrying out hydrolytic reaction and ester interchange reaction of specific edible vegetable fats and oils using a 1,3-regiospecific lipase.
CONSTITUTION: A 1,3-regiospecific lipase, derived from the genus Rhizopus, etc., and used for reaction, together with an enzyme activator such as lecithin, is dissolved in water and immobilized on a carrier such as Celite(R) or an ion exchange resin to prepare an immobilized enzyme agent, which is then filled in a column. Mixed fats and oils (substrate) of edible liquid fats and oils such as olive oil, having the moisture content therein regulated within the range of 100 to 800ppm, preferably 200 to 400ppm and containing ≥40% oleic acid at the 2-position of triglyceride and palm oil under the same conditions as described above are continuously passed through the column to carry out hydrolytic reaction and ester interchange reaction. Thereby, since the resultant fats and oils are excellent in emulsifying characteristics and temperature shock resistance without requiring tempering, margarines, etc., can efficiently be produced.
NAKAJIMA TAKAKO
MIYAGAWA HISAO
KATOU CHIHIRO
