Title:
G PROTEINS
Document Type and Number:
Japanese Patent JP2022084574
Kind Code:
A
Abstract:
To provide a mutant of a heterotrimeric G protein alpha (Gα) subunit, which is useful for parental ligand binding screening, assay development, and so on.SOLUTION: The invention provides a mutant of a parent heterotrimeric G protein alpha (Gα) subunit, where the mutant (i) lacks at least one helix of the helical domain of the parent Gα subunit; (ii) is capable of binding to a GPCR in the absence of a heterotrimeric G protein beta (Gβ) subunit and a heterotrimeric G protein gamma (Gγ) subunit; and (iii) has an amino acid sequence that contains one or more mutations compared to the amino acid sequence of the parent heterotrimeric Gα subunit, the mutations selected from a deletion, a substitution and an insertion.SELECTED DRAWING: None
More Like This:
Inventors:
BYRON CARPENTER
ANDREW LESLIE
RONY NEHME
CHRISTOPHER GORDON TATE
ANTONY WARNE
ANDREW LESLIE
RONY NEHME
CHRISTOPHER GORDON TATE
ANTONY WARNE
Application Number:
JP2022007787A
Publication Date:
June 07, 2022
Filing Date:
January 21, 2022
Export Citation:
Assignee:
HEPTARES THERAPEUTICS LTD
International Classes:
C12N15/55; A61K38/16; A61K39/395; A61P35/00; C07K14/47; C07K16/28; C07K17/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/16; C12N15/12; C12N15/63; C12Q1/68; G01N33/15; G01N33/50
Foreign References:
| WO1998016557A1 | 1998-04-23 | |||
| WO2004092199A2 | 2004-10-28 |
Other References:
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. VOL: 282, NR: 47, JPN5019001285, 23 December 2007 (2007-12-23), US, pages 34019 - 34030, ISSN: 0005017410
Attorney, Agent or Firm:
Patent Business Corporation Shinei Patent Office