Title:
GEL ELECTROPHORESIS METHOD
Document Type and Number:
Japanese Patent JPH0634602
Kind Code:
A
Abstract:
PURPOSE: To separate/analyse protein, peptide or nucleic acid of which molecular weight widely ranges from 1,000 to hundreds of thousands, with high accuracy, resolution and reproducibility by using a kind of polyacrylamide gel.
CONSTITUTION: As for gel electrophoresis method for protein, peptide or nucleic acid of which molecular weight ranges from 1,000 to hundreds of thousands, polycrylamide gel with concentration gradient of 2-25% is used for gel electrophoresis of protein, peptide or nucleic acid. One butter solution of tris-tricine mixture, tris-bicine mixture, tris-2-(N-morpholino) ethanesulfonic acid, and tris- N-(acetoamide)-2-aminoethanesulfonic acid is used for electrophoresis buffer solution.
Inventors:
UDAGAWA AKIKO
NOZU KAZUAKI
SHIRATORI MIEKO
EHATA YORIYOSHI
NOZU KAZUAKI
SHIRATORI MIEKO
EHATA YORIYOSHI
Application Number:
JP19218692A
Publication Date:
February 10, 1994
Filing Date:
July 20, 1992
Export Citation:
Assignee:
DAIICHI PURE CHEMICALS CO LTD
International Classes:
B01D57/02; C07K1/26; G01N27/447; (IPC1-7): G01N27/447; B01D57/02; C07K3/14
Attorney, Agent or Firm:
Miyuki Ariga (2 outside)
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