Title:
GENE OF PROTEIN HAVING LIPASE ACTIVITY
Document Type and Number:
Japanese Patent JPH02299588
Kind Code:
A
Abstract:
PURPOSE: To obtain the subject gene useful to be integrated into an expression vector, etc., to form a vector for the production of lipase by extracting and purifying an mRNA from a lipase-producing cell of Ceotrichum candidum and cloning a lipase gene with the mRNA.
CONSTITUTION: An expression vector is prepared by integrating a lipase cDNA into a vector of E.coli, yeast, Bacillus subtilis, etc., and inserting a proper promoter to the upstream of the cDNA. The promoter is preferably tryptophan β-galactosidase, alkaline phosphatase, etc., for E.coli, acidic phosphatase, alcohol dehydrogenase, etc., for yeast and α-amylase, protease, etc., for Bacillus subtilis. A cultured cell such as COS cell may be used as the host.
Inventors:
SHIMADA YUJI
TOMINAGA YOSHIO
SUGIHARA AKIO
IIIZUMI TARO
TOMINAGA YOSHIO
SUGIHARA AKIO
IIIZUMI TARO
Application Number:
JP7472189A
Publication Date:
December 11, 1990
Filing Date:
March 27, 1989
Export Citation:
Assignee:
KURITA WATER IND LTD
OSAKA CITY
OSAKA CITY
International Classes:
C12N9/20; C12N15/09; C12N15/55; C12R1/645; (IPC1-7): C12N9/20; C12N15/55