Title:
無血清培地を用いた遺伝子導入方法
Document Type and Number:
Japanese Patent JP3754090
Kind Code:
B2
Abstract:
A method for transfening a gene into target cells by a retrovirus with the use of a serum-free medium. This method comprises infecting target cells with a retrovirus in a serum-free medium optionally containing low-density lipoprotein and/or cytokines in the presence of a functional substance such as fibronectin in an amount effective in elevating the gene transfer efficiency of the retrovirus into the target cells when the retrovirus and the target cells are present together.
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Inventors:
Bagni, Claude
Amber, Anne-Marie
Manoni, Patrice
Amber, Anne-Marie
Manoni, Patrice
Application Number:
JP50964599A
Publication Date:
March 08, 2006
Filing Date:
July 15, 1998
Export Citation:
Assignee:
Takara Bio Inc.
International Classes:
C12N15/09; A61K48/00; C12N5/10; C12N7/00; C12N15/00; C12N15/48; C12N15/63; C12N15/86; C12N15/867
Foreign References:
| WO1995026200A1 | ||||
| WO1997018318A1 |
Other References:
Human Gene Therapy, 1996年,Vol.7, p.33-38
Attorney, Agent or Firm:
Aoyama Aoi
Mitsuo Tanaka
Mitsuo Tanaka