Title:
ＨｂＡ１ｃデヒドロゲナーゼ
Document Type and Number:
Japanese Patent JP7471772
Kind Code:
B2
Abstract:
This invention provides an HbAlc dehydrogenase that is capable of directly acting on hemoglobin Alc and is less likely to be influenced by oxygen concentration and a method for measurement and a kit of assay reagents using such HbAlc dehydrogenase. The HbAlc dehydrogenase having dehydrogenase activity and capable of directly acting on HbAlc is obtained by substitution of one or more amino acid residues at positions corresponding to positions 280, 269, 54, 241, and 267 of the amadoriase that is capable of directly acting on hemoglobin Alc and is derived from, for example, the genus Coniochaeta. This invention also provides a method for measurement of HbAlc, a kit of assay reagents, and a sensor using such HbAlc dehydrogenase. Such HbAlc dehydrogenase is capable of directly acting on hemoglobin Alc and has lowered oxidase activity and/or enhanced dehydrogenase activity. This not only eliminates the need for treatment of hemoglobin Alc with a protease but also enables the use of an electron mediator in the measurement of HbAlc, thereby reducing effects due to oxygen concentration, and enables HbAlc measured with high sensitivity.
Inventors:
Atsushi Ichiyanagi
Application Number:
JP2018513229A
Publication Date:
April 22, 2024
Filing Date:
April 21, 2017
Export Citation:
Assignee:
Kikkoman Corporation
International Classes:
C12N9/04; C12M1/34; C12Q1/00; C12Q1/32
Domestic Patent References:
| JP2013500729A |
Foreign References:
| WO2004038034A1 | ||||
| WO2015060429A1 |
Attorney, Agent or Firm:
Patent Attorney Corporation Hiraki International Patent Office