Title:
化学的に修飾されたガイドＲＮＡを使用する高特異性ゲノム編集
Document Type and Number:
Japanese Patent JP7093728
Kind Code:
B2
Abstract:
The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.
Inventors:
Dellinger, Douglas Jay
Ryan, Daniel Yee
Suba Deep, Roy
Jeffrey Earl, Sampson
Ryan, Daniel Yee
Suba Deep, Roy
Jeffrey Earl, Sampson
Application Number:
JP2018564259A
Publication Date:
June 30, 2022
Filing Date:
June 08, 2017
Export Citation:
Assignee:
AGILENT TECHNOLOGIES, INC.
International Classes:
C12N15/09; C12N15/113; C12Q1/6834; C12Q1/6837; C40B10/00
Domestic Patent References:
| JP2015523856A | ||||
| JP2017537626A |
Foreign References:
| WO2015026885A1 |
Other References:
Nature Biotechnology (2015) Vol.33, No.9, pp.985-991
MEGHDAD RAHDAR, et al.,"Synthetic CRISPR RNA-Cas9-guided genome editing in human cells",PNAS (2015), E7110-E7117
MEGHDAD RAHDAR, et al.,"Synthetic CRISPR RNA-Cas9-guided genome editing in human cells",PNAS (2015), E7110-E7117
Attorney, Agent or Firm:
Okuyama Shoichi
Tetsuo Matsushima
Nakamura Ayako
Soji Morimoto
Yu Tanaka
Koichi Tokumoto
Akiko Mizushima
Kohei Arihara
Tetsuo Matsushima
Nakamura Ayako
Soji Morimoto
Yu Tanaka
Koichi Tokumoto
Akiko Mizushima
Kohei Arihara