Title:
高温でリボヌクレアーゼを失活する方法
Document Type and Number:
Japanese Patent JP2006524504
Kind Code:
A
Abstract:
Methods for protecting RNA from RNase degradation and for inactivating RNases in solution are disclosed. The invention includes methods for protecting RNA during storage, for performing quantitative PCR reactions, or for preparing cDNA. The method includes using a combination of an RNase inhibitor in a solution containing or devoid of reducing agents and high heat to render RNases inactive.
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Inventors:
Hoang, Feng
Andrews, Christine
Schulz, John
Andrews, Christine
Schulz, John
Application Number:
JP2006507196A
Publication Date:
November 02, 2006
Filing Date:
March 15, 2004
Export Citation:
Assignee:
Promega Corporation
International Classes:
C12N15/00; C12N9/99; C12N15/09; C12N15/10; C12P19/34; C12Q1/68
Other References:
JPN6010012598, Ｔｅｃｈｎｏｔｅｓ． ［ｏｎｌｉｎｅ］． ２００１． Ｖｏｌ．８， Ｎｏ．２ ［Ｒｅｔｒｉｅｖｅｄ ｏｎ ２０１０−０３−０３］． Ｒｅｔｒｉｅｖｅｄ ｆｒｏｍ ｔｈｅ ｉｎｔｅｒｎｅｔ：＜ＵＲＬ：
JPN6010057343, Ｐｒｏｔｅｉｎ ｓｃｉｅｎｃｅ． １９９９， Ｖｏｌ．８， Ｎｏ．２， ｐ．４３０−４３４
JPN6010057343, Ｐｒｏｔｅｉｎ ｓｃｉｅｎｃｅ． １９９９， Ｖｏｌ．８， Ｎｏ．２， ｐ．４３０−４３４
Attorney, Agent or Firm:
Tadahiko Ito
Shinsuke Onuki
Tadashige Ito
Shinsuke Onuki
Tadashige Ito