PURPOSE: To obtain the subject plant having efficiently transduced characters such as disease resistance and treading resistance of plant belonging to the genus Zoysia except Zoysia japonica Steud. by subjecting protoplast derived from Zoysia japonica Steud. and protoplast of another plant belonging to the genus Zoysia to cell fusion.
CONSTITUTION: Protoplast prepared from cells or a plant derived from Zoysia japonica Steud. and protoplast prepared from cells, plant or seeds of another plant belonging to the genus Zoysia are fused preferably by electric treatment to give the objective plant. The protoplast is preferably carried out by treating cells, a plant, etc., in an enzyme solution containing cellulase, etc., at 16-30°C at 0-50rpm for 3-18 hours.
KURODA HIROFUMI
YOSHIDA KAORU
SHINOZAKI SATOSHI
