Title:
【発明の名称】疾患の臨床的発現に基づく、診断用試薬を得る方法、アッセイ、及び治療剤
Document Type and Number:
Japanese Patent JP3135126
Kind Code:
B2
Abstract:
Antibodies are recovered and isolated from the sera of a number "n" of patients each of which have or have had the same given disease so that each of the "n" patients has, within a large number of antibodies, some antibodies uniquely associated with the same disease of interest. The antibodies of a first patient are bound to a support surface. To carry out initial screening libraries of molecules, such as peptides displayed on the surface of bacteriophage, are brought into contact with the bound antibodies of the first patient under conditions where binding will occur. Unbound molecules (peptides) are washed away and bound molecules (peptides) on the bacteriophage are identified. Secondary screening is then carried out by extracting and labeling the antibodies of a second patient and using the labeled antibodies to probe the molecules (peptides) isolated in the initial screening.
Inventors:
Santi Daniel buoy.
Application Number:
JP51459495A
Publication Date:
February 13, 2001
Filing Date:
November 17, 1994
Export Citation:
Assignee:
Santi Daniel buoy.
International Classes:
C07K1/04; C07K16/00; C07K16/06; C07K16/10; G01N33/531; C07K16/34; C12Q1/70; C40B30/04; G01N33/543; G01N33/569; G01N33/68; (IPC1-7): G01N33/543; C12Q1/70; G01N33/531; G01N33/569
Domestic Patent References:
| JP8506493A | ||||
| JP62501314A |
Other References:
【文献】米国特許5270170(US,A)
【文献】国際公開91/19818(WO,A1)
【文献】国際公開91/19818(WO,A1)
Attorney, Agent or Firm:
Hatsushi Shimizu