Title:
増加的切断短縮化核酸及びその製造方法
Document Type and Number:
Japanese Patent JP2004528801
Kind Code:
A
Abstract:
A series of methods that utilize the incremental truncation of nucleic acids are described to create a plurality of modified nucleic acids and hybrid polypeptides. A plurality of substantially all possible single base-pair deletions of a given nucleic acid sequence is created. A method of making shuffled incremental truncated nucleic acids, which is independent of nucleic acid sequence homology, is also described. These methods can be used in protein engineering, protein folding, protein evolution, and the chemical synthesis of novel hybrid proteins and polypeptides.
Inventors:
Benkovic Stephen Jay
Ostermeier Mark
Rats Stefan
Nixon Andrew Y
Ostermeier Mark
Rats Stefan
Nixon Andrew Y
Application Number:
JP2001573030A
Publication Date:
September 24, 2004
Filing Date:
November 16, 2000
Export Citation:
Assignee:
The Penn State Research Foundation
International Classes:
C12N15/09; C07K14/00; C12N15/01; C12N15/10; (IPC1-7): C12N15/09; C12N15/01
Attorney, Agent or Firm:
Minoru Nakamura
Fumiaki Otsuka
Sadao Kumakura
Shishido Kaichi
Hideto Takeuchi
Toshio Imajo
Nobuo Ogawa
Village shrine Atsuo
Takaki Nishijima
Atsushi Hakoda
Fumiaki Otsuka
Sadao Kumakura
Shishido Kaichi
Hideto Takeuchi
Toshio Imajo
Nobuo Ogawa
Village shrine Atsuo
Takaki Nishijima
Atsushi Hakoda