Title:
ヒト胚性幹細胞(hESC)株の樹立のための内部細胞塊の単離
Document Type and Number:
Japanese Patent JP2005514004
Kind Code:
A
Abstract:
A method of establishing a cell line from the inner cell mass of a blastocyst, comprising: isolating a blastocyst having a zona pellucida, a trophectoderm, and an inner cell mass; creating an aperture in the blastocyst by laser ablation; isolating cells from the inner cell mass from the blastocyst through the aperture; and culturing the cells to establish a cell line, wherein the cells are cultured under feeder free conditions.
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Inventors:
Paris, Filza Lajes
Totei, Satish Mahade Olao
Saxena, Shairaja Anupam
Totei, Satish Mahade Olao
Saxena, Shairaja Anupam
Application Number:
JP2003523634A
Publication Date:
May 19, 2005
Filing Date:
August 20, 2002
Export Citation:
Assignee:
Reliance Life Sciences P.V.T., LDT
International Classes:
C12N5/071; A61B17/435; A61B18/20; A61K35/54; C12N5/07; C12N5/0735; C12N13/00; (IPC1-7): C12N5/06
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita