To provide a laser scanning microscope for fluorescence analysis which does not require an emission filter.

In the laser scanning microscope for fluorescence analysis, radiation paths for illumination and detection are optically coupled by a dichroic main color splitter (1), and detected light from a sample is guided to a plurality of detectors by another beam splitter (2). The incident angle of illumination light and/or the incident angle of the light from the sample on the surface of at least the main color splitter or at least another splitter is smaller than 45 degrees.