Title:
バキュロウイルスベクターを使用して核酸分子を胚性幹細胞に送達する方法
Document Type and Number:
Japanese Patent JP5006312
Kind Code:
B2
Abstract:
There is provided a method of delivering a nucleic acid molecule to an embryonic stem cell, including a human embryonic stem cell, by infecting the embryonic stem cell with a baculoviral vector comprising the nucleic acid molecule. Embryonic stem cells transduced by this method are useful for treating a disease or disorder in a subject.
Inventors:
One, Shu
Jen, Jemin
Jen, Jemin
Application Number:
JP2008513434A
Publication Date:
August 22, 2012
Filing Date:
May 26, 2006
Export Citation:
Assignee:
AGENCY FOR SCIENCE,TECHNOLOGY AND RESEARCH
International Classes:
C12N15/09; A61K35/12; A61K48/00; A61P1/00; A61P1/16; A61P1/18; A61P3/10; A61P9/00; A61P11/00; A61P13/12; A61P21/00; A61P21/04; A61P25/00; A61P25/16; A61P25/28; A61P27/02; A61P35/00; A61P35/02; A61P43/00; C12N5/0735
Foreign References:
| WO2000077233A1 | ||||
| WO2003056019A1 |
Other References:
PALOMBO,F. et al.,Site-specific integration in mammalian cells mediated by a new hybrid baculovirus-adeno-associated virus vector.,J. Virol.,1998年 6月,Vol.72, No.6,pp.5025-34
PFEIFER,A. et al.,Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos.,Proc. Natl. Acad. Sci. USA.,2002年 2月19日,Vol.99, No.4,pp.2140-5
STEVENSON,A. et al.,Herpesvirus saimiri-based gene delivery vectors maintain heterologous expression throughout mouse embryonic stem cell differentiation in vitro.,Gene Ther.,2000年 3月,Vol.7, No.6,pp.464-71
PFEIFER,A. et al.,Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos.,Proc. Natl. Acad. Sci. USA.,2002年 2月19日,Vol.99, No.4,pp.2140-5
STEVENSON,A. et al.,Herpesvirus saimiri-based gene delivery vectors maintain heterologous expression throughout mouse embryonic stem cell differentiation in vitro.,Gene Ther.,2000年 3月,Vol.7, No.6,pp.464-71
Attorney, Agent or Firm:
Satoshi Kono
Makoto Nakamura
Kurata Masatoshi
Takashi Mine
Yoshihiro Fukuhara
Toshio Shirane
Sadao Muramatsu
Nobuhisa Nogawa
Katsu Sunagawa
Ryo Hashimoto
Tetsuya Kazama
Makoto Nakamura
Kurata Masatoshi
Takashi Mine
Yoshihiro Fukuhara
Toshio Shirane
Sadao Muramatsu
Nobuhisa Nogawa
Katsu Sunagawa
Ryo Hashimoto
Tetsuya Kazama