Title:
METHOD FOR DETECTING NUCLEIC ACID AMPLIFICATION PRODUCT BY FLUORESCENCE POLARIZATION
Document Type and Number:
Japanese Patent JP2002272475
Kind Code:
A
Abstract:
To provide a method for detecting a nucleic acid amplification product by the fluorescence polarization.
This method includes the steps of adding a fluorescence-labelled primer that hybridizes to a single-stranded loop part to a nucleic acid amplification product obtained by the loop-mediated isothermal amplification(LAMP) reaction and measuring a fluorescence-polarization degree of the reaction mixture.
Inventors:
MASUBUCHI HARUMI
NOUTOMI TSUGUNORI
NOUTOMI TSUGUNORI
Application Number:
JP2001083428A
Publication Date:
September 24, 2002
Filing Date:
March 22, 2001
Export Citation:
Assignee:
EIKEN CHEMICAL
International Classes:
G01N33/53; C12N15/09; C12Q1/68; G01N33/566; (IPC1-7): C12N15/09; C12Q1/68; G01N33/53; G01N33/566
Domestic Patent References:
| JP2000217600A | 2000-08-08 | |||
| JPH1146771A | 1999-02-23 | |||
| JPH09187275A | 1997-07-22 | |||
| JPH0838199A | 1996-02-13 | |||
| JP2000019172A | 2000-01-21 |
Foreign References:
| WO2000028082A1 | 2000-05-18 |
Attorney, Agent or Firm:
Yusuke Hiraki (1 person outside)
Previous Patent: METHOD FOR TESTING SQUAMOUS CELL
Next Patent: PROTEIN-EXPRESSING SYSTEM THAT USES PLANT BODY
Next Patent: PROTEIN-EXPRESSING SYSTEM THAT USES PLANT BODY