To provide a method for detecting a nucleic acid sequence in a specimen quickly, easily and in a good reproducibility.

This method for detecting the nucleic acid sequence by performing an amplifying reaction by using an amplifying oligonucleotide bonded with a first ligand in a specimen solution containing the specific nucleic acid sequence and bringing a bonded material bonded with a second ligand specifically capable of bonding with the nucleic acid sequence in contact with the above at the same time with the amplification reaction or after the reaction and detecting a complex bonded with a bonded second ligand comprises a process of bonding the second ligand part of the complex on the surface of a liquid-passing filter bonded with a second ligand-capturing agent, a process of dripping a solution of a labeled physiologically active substance capable of bonding with the first ligand for bonding the labeled physiologically active substance with the first ligand and a process for titrating the label bonded with the liquid-passing filter.